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1. Add 50 ml of tissue culture water to 5 mg vial of poly-D-lysine to create a working stock of 0.1 mg/ml.
2. Dilute working stock of poly-D-lysine 1:10 to 0.01 mg/ml in tissue culture water.
3. Add diluted poly-D-lysine to plate and incubate for 20 minutes at room temperature in the tissue culture hood.
Guide:
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3 ml for 100 mm dish
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1.5 ml for 60 mm dish
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1 ml for 6-well plate
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0.5 ml for 24-well plate
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0.2 ml for 48-well plate
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0.05 ml for 96-well plate
4. Wash the plate once with tissue culture water twice and allow to air dry in the hood for 1 – 2 hours.
Reagents:
Poly-D-Lysine hydrobromide; Sigma Cat #P6407-5MG
Tissue culture dish, 100 mm, Falcon® Cat #353003
Tissue culture dish, 60 mm, TPP® Cat #93060
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